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The effect of chitin and chitosan on the proliferation of human skin fibroblasts and keratinocytes in vitro

Paper ID Volume ID Publish Year Pages File Format Full-Text
13564 881 2001 8 PDF Available
Title
The effect of chitin and chitosan on the proliferation of human skin fibroblasts and keratinocytes in vitro
Abstract

The effects of chitin [(1→4)-2-acetamido-2-deoxy-β-d-glucan] and its partially deacetylated derivatives, chitosans, on the proliferation of human dermal fibroblasts and keratinocytes were examined in vitro. Chitosans with relatively high degrees of deacetylation strongly stimulated fibroblast proliferation while samples with lower levels of deacetylation showed less activity. Fraction, CL313A, a shorter chain length, 89% deacetylated chitosan chloride was further evaluated using cultures of fibroblasts derived from a range of human donors. Some fibroblast cultures produced a positive mitogenic response to CL313A treatment with proliferation rates being increased by approximately 50% over the control level at an initial concentration of 50 μg/ml, whilst others showed no stimulation of proliferation or even a slight inhibition (<10%). The stimulatory effect on fibroblast proliferation required the presence of serum in the culture medium suggesting that the chitosan may be interacting with growth factors present in the serum and potentiating their effect. In contrast to the stimulatory effects on fibroblasts, fraction CL313A inhibited human keratinocyte mitogenesis with up to 40% inhibition of proliferation being observed at 50 μg/ml. In general highly deacetylated chitosans were more active than those with a lower degree of deacetylation. These data demonstrate that highly deacetylated chitosans can modulate human skin cell mitogenesis in vitro. Analysis of their effects on cells in culture may be useful as a screen for their potential activity in vivo as wound healing agents, although in the case of fibroblasts it is important to select appropriate strains of cells for use in the screen.

Keywords
Chitosan; Chitin; Fibroblast; Keratinocyte; Skin; Wound healing
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The effect of chitin and chitosan on the proliferation of human skin fibroblasts and keratinocytes in vitro
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 22, Issue 22, 15 November 2001, Pages 2959–2966
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us