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Hollow fiber membrane diffusive permeability regulates encapsulated cell line biomass, proliferation, and small molecule release

Paper ID Volume ID Publish Year Pages File Format Full-Text
13579 882 2002 11 PDF Available
Title
Hollow fiber membrane diffusive permeability regulates encapsulated cell line biomass, proliferation, and small molecule release
Abstract

Using histological and HPLC methods, we examined the influence of hollow fiber membrane transport properties on encapsulated PC12 cell biomass, proliferation and the release of dopamine over 4 weeks in culture. Our data indicated that encapsulated cell biomass, the number of proliferating cells, and the quantity of dopamine released increased as a function of increasing hollow fiber encapsulation membrane diffusive permeability. Overall the percentage of viable cells and the biomass architecture, however, was not significantly affected by differences in membrane transport. When compared to membrane sieving properties, membrane diffusive transport and membrane hydraulic permeability were better indictators of biomass size, proliferating cell number, and dopamine release from encapsulated cells. Studies examining the sustained release of DA from membranes of differing permeability suggest that membrane diffusive permeability can be used to regulate the quantity of small molecules released per unit time at steady state, and should be considered when dosing is an important determinant of implant efficacy.

Keywords
Encapsulation technology; Transport characterization; Poly(acrylonitrile-co-vinylchloride) (PAN-PVC); PC12 cells; Dopamine release
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Hollow fiber membrane diffusive permeability regulates encapsulated cell line biomass, proliferation, and small molecule release
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 23, Issue 24, December 2002, Pages 4689–4699
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us