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Activated polyurethane modified with latent thiol groups ☆

Paper ID Volume ID Publish Year Pages File Format Full-Text
13585 882 2002 6 PDF Available
Title
Activated polyurethane modified with latent thiol groups ☆
Abstract

A novel type of modified polyurethane with pendant acetylthio groups (as a latent form of thiol groups) has been proposed for the use in surface modifications with various biomolecules. The polymer was prepared via a modified variant of low-temperature bromoalkylation of urethane hard segments followed by the reaction of pendant bromoalkyl groups with thiolacetic acid in mild conditions. The extent of modification with acetylthio groups can be made as high as 0.45 mmol/g. After deprotection of acetylthio groups and reaction of the resulting thiol groups with an excess of Ellman's reagent, 0.1 nmol/cm2 of thiol-reactive 3-carboxy-4-nitrophenyldithio groups were detected on the surface of films cast from the modified polymer. A sensitive fluorescent probe—dansyl-l-cysteine was used for the quantification of thiol-reactive groups bound to the surface. The acetylthio-modified polyurethane is sufficiently stable to withstand conditions typical for the high-temperature processing (molding, extrusion) of polyurethanes.

Keywords
Polyurethane; Thiols; Immobilization; Biomaterials
First Page Preview
Activated polyurethane modified with latent thiol groups ☆
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 23, Issue 24, December 2002, Pages 4753–4758
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering