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Growth of human endothelial cells on photochemically grafted Gly–Arg–Gly–Asp (GRGD) chitosans

Paper ID Volume ID Publish Year Pages File Format Full-Text
13591 882 2002 7 PDF Available
Title
Growth of human endothelial cells on photochemically grafted Gly–Arg–Gly–Asp (GRGD) chitosans
Abstract

To improve ECs adhesion and growth on chitosan, cell adhesive peptide Gly–Arg–Gly–Asp (GRGD) was photochemically grafted to its surface. Grafting 0.025 m of GRGD-SANPAH (N-Succinimidyl-6-[4′-azido-2′-nitrophenylamino]-hexanoate) solutions to chitosan and tripolyphosphate anhydrous crosslinked chitosan (chitosan-TPP) surfaces was performed by surface adsorption of the peptide and subsequent ultraviolet (UV) irradiation for photoreaction. FTIR spectra and electron spectroscopy for chemical analysis (ESCA) confirmed that GRGD was successfully grafted to form chitosan-GRGD surfaces (e.g., the composition fraction of nitrogen (N1s) for chitosan-GRGD surface was 5.2% instead of 3.2% for chitosan only). Moreover, the grafting efficiencies for 0.025 m of GRGD to chitosan and chitosan-TPP surfaces were about 83% and 53%, semi-quantitatively analyzed by an HPLC. Human umbilical vein ECs were well adhered and grew on chitosan-GRGD and chitosan-TPP–GRGD surfaces after 36 h of incubation but not chitosan and chitosan-TPP ones. Moreover, the viability for the growth of ECs on the chitosan-GRGD and chitosan-TPP–GRGD surfaces analyzed by MTT assay also confirmed the efficacy of the GRGD immobilization. In conclusion, the chitosan-GRGD and chitosan-TPP–GRGD surfaces were prepared by a photochemical technique that could enhance the adhesion and growth of ECs on those surfaces for further applications for tissue engineering.

Keywords
GRGD grafted chitosan; Growth of human ECs
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Growth of human endothelial cells on photochemically grafted Gly–Arg–Gly–Asp (GRGD) chitosans
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 23, Issue 24, December 2002, Pages 4803–4809
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us