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Coating with genetic engineered hydrophobin promotes growth of fibroblasts on a hydrophobic solid

Paper ID Volume ID Publish Year Pages File Format Full-Text
13597 882 2002 8 PDF Available
Title
Coating with genetic engineered hydrophobin promotes growth of fibroblasts on a hydrophobic solid
Abstract

Class I Hydrophobins self-assemble at hydrophilic–hydrophobic interfaces into a highly insoluble amphipathic film. Upon self-assembly of these fungal proteins hydrophobic solids turn hydrophilic, while hydrophilic materials can be made hydrophobic. Hydrophobins thus change the nature of a surface. This property makes them interesting candidates to improve physio- and physico-chemical properties of implant surfaces. We here show that growth of fibroblasts on Teflon can be improved by coating the solid with genetically engineered SC3 hydrophobin. Either deleting a stretch of 25 amino acids at the N-terminus of the mature hydrophobin (TrSC3) or fusing the RGD peptide to this end (RGD-SC3) improved growth of fibroblasts on the solid surface. In addition, we have shown that assembled SC3 and TrSC3 are not toxic when added to the medium of a cell culture of fibroblasts in amounts up to 125 μg ml−1.

Keywords
Biocompatibility; RGD peptide; Surface modification; Self-assembly; Hydrophobin
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Coating with genetic engineered hydrophobin promotes growth of fibroblasts on a hydrophobic solid
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 23, Issue 24, December 2002, Pages 4847–4854
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us