fulltext.study @t Gmail

Core–sheath structured fibers with pDNA polyplex loadings for the optimal release profile and transfection efficiency as potential tissue engineering scaffolds

Paper ID Volume ID Publish Year Pages File Format Full-Text
1361 82 2011 11 PDF Available
Title
Core–sheath structured fibers with pDNA polyplex loadings for the optimal release profile and transfection efficiency as potential tissue engineering scaffolds
Abstract

Emulsion electrospinning was initially applied to prepare core–sheath structured fibers with a core loading of pDNA or pDNA polyplexes inside a fiber sheath of poly(dl-lactide)–poly(ethylene glycol) (PELA). The inclusion of poly(ethylene imine) (PEI) and poly(ethylene glycol) (PEG) were expected to modulate the release profiles and achieve a balance between cytotoxicity and transfection efficiency. The core–sheath fibers enhance the structural integrity and maintain the biological activity of pDNA during the electrospinning process, incubation in release buffer and enzyme digestion. The addition of hydrophilic PEI into the fiber matrix accelerates pDNA release, while the encapsulation of pDNA polyplexes within the fibers led to no further release after an initial burst. However, sustained release of pDNA polyplexes has been achieved through PEG incorporation, and the effective release lifetime can be controlled between 6 and 25 days, dependent on the amount loaded and the molecular weight of PEG. Higher N/P ratios of PEI to DNA result in lower cell attachment, while cell viability is dependent on the effective concentration of pDNA polyplexes released from the fibers. While no apparent transfection is detected for pDNA-loaded PELA fibers, PEG incorporation into fibers containing pDNA polyplexes leads to over an order of magnitude increase in the transfection efficiency. pDNA polyplex-loaded fibers containing 10% PEG show the best performance in balancing transfection efficiency and cell viability. It is suggested that electrospun core–sheath fibers integrated with DNA condensation techniques provide the potential to produce inductive tissue engineering scaffolds able to manipulate the desired signals at effective levels within the local tissue microenvironment.

Keywords
Emulsion electrospinning; Core–sheath structured fiber; pDNA polyplexes; Release modulation; Transfection efficiency
First Page Preview
Core–sheath structured fibers with pDNA polyplex loadings for the optimal release profile and transfection efficiency as potential tissue engineering scaffolds
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Acta Biomaterialia - Volume 7, Issue 6, June 2011, Pages 2533–2543
Authors
, , , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us