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Europium-doped bioapatite: a new photostable biological probe, internalizable by human cells

Paper ID Volume ID Publish Year Pages File Format Full-Text
13627 885 2003 7 PDF Available
Title
Europium-doped bioapatite: a new photostable biological probe, internalizable by human cells
Abstract

The authors prepared at low temperatures (37°C) a novel inorganic bioprobe. It consisted of mineral nanoparticles of apatitic tricalcium phosphate doped with europium, of size, structure and composition close to those of the mineral part of calcified tissues. In contrast to organic probes which degrade rapidly (photobleaching), the red luminescence of the new probe is photostable. Moreover, this luminescence can be obtained under visible irradiation, which makes it suitable for prolonged examination of live cells.Human pancreatic epithelial cells in culture were incubated with these particles and their internalization was observed by laser scanning confocal microscopy. Transmission electron microscopy and electron microdiffraction analysis confirmed that the particles were internalized retaining their original apatitic structure. This probe may thus be of value for biovectorization.

Keywords
Calcium phosphate; Luminescent probe; Europium; Internalization; Human cells
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Europium-doped bioapatite: a new photostable biological probe, internalizable by human cells
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 24, Issue 19, August 2003, Pages 3365–3371
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us