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In vitro reconstructed dermis implanted in human wounds: degradation studies of the HA-based supporting scaffold

Paper ID Volume ID Publish Year Pages File Format Full-Text
13653 888 2000 9 PDF Available
Title
In vitro reconstructed dermis implanted in human wounds: degradation studies of the HA-based supporting scaffold
Abstract

The objective of the present study was to demonstrate the safety and efficacy of a dermal replacement for cutaneous wounds of diverse origin. Autologous fibroblasts were cultured in fleece scaffolds made from benzyl esters of hyaluronic acid and applied onto cutaneous lesions. The cases presented are (1) skin removal for multiple epithelioma and (2) chronic deep decubitus ulcer. Dermal-like tissue applied by the surgeon elicited no adverse reactions, and was fully integrated and well-vascularized by 1–3 weeks. In Case 1, the material was fully integrated after 1 week, and after 3 weeks an epidermal autograft was overlaid which showed good take with excellent integration observed after 4 weeks. At 12 months, skin demonstrated visual normo-elastic properties and no signs of excessive scarring. In Case 2, 2–3 weeks after the dermal implant was applied, the wound was invaded with granulation tissue and healing occurred by secondary intention. The ulcer was healed by 8 weeks, with the biomaterial completely resorbed and a complete re-epithelialization over the dermal-like tissue. These results suggest that autologous fibroblast culture in hyaluronan-derived scaffolds may be successfully grafted in diverse cutaneous pathologies and constitute a suitable bed for further epidermal implantation.

Keywords
CEA: cultured epithelial autograft; NWM: non-woven mesh; DMEM: dulbecco modified eagle's medium; ECM: extracellular matrixChronic ulcers; Surgical wounds; Artificial dermis; Hyaluronan esters
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In vitro reconstructed dermis implanted in human wounds: degradation studies of the HA-based supporting scaffold
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 21, Issue 21, 1 November 2000, Pages 2183–2191
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us