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Chronic exposure of human ovarian carcinoma cells to free or HPMA copolymer-bound mesochlorin e6 does not induce P-glycoprotein-mediated multidrug resistance

Paper ID Volume ID Publish Year Pages File Format Full-Text
13655 888 2000 8 PDF Available
Title
Chronic exposure of human ovarian carcinoma cells to free or HPMA copolymer-bound mesochlorin e6 does not induce P-glycoprotein-mediated multidrug resistance
Abstract

The acquisition of multidrug resistance in human ovarian carcinoma A2780 cells was investigated after chronic exposure to free mesochlorin e6 monoethylenediamine (Mce6) and N-(2-hydroxypropyl)methacrylamide (HPMA) copolymer-bound Mce6 (P(GG)-Mce6). The dose that inhibits growth by 50% (IC50) was determined for free Mce6 (2.09±0.32 μm) and P(GG)-Mce6 (204.15±28.97 μm) to utilize similar effective doses of drug. A total of 14 drug exposures were performed over a period of 78 days. Cells were characterized by IC50 dose, MDR1 gene expression and anti-human P-glycoprotein (P-gp) antibody binding after each drug exposure. At the conclusion of the experiment, neither the A2780 cells habitually exposed to free Mce6 or P(GG)-Mce6 were significantly different than the control A2780 cells indicating cells did not acquire a MDR phenotype. The doxorubicin (DOX)-resistant A2780/AD cells served as a positive control.

Keywords
N-(2-hydroxypropyl)methacrylamide (HPMA); Mesochlorin e6 monoethylenediamine (Mce6); Multidrug resistance (MDR); P-glycoprotein (P-gp)
First Page Preview
Chronic exposure of human ovarian carcinoma cells to free or HPMA copolymer-bound mesochlorin e6 does not induce P-glycoprotein-mediated multidrug resistance
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 21, Issue 21, 1 November 2000, Pages 2203–2210
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering