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Electrophysiological recordings of patterned rat brain stem slice neurons

Paper ID Volume ID Publish Year Pages File Format Full-Text
13662 889 2002 8 PDF Available
Title
Electrophysiological recordings of patterned rat brain stem slice neurons
Abstract

Dissociated neuronal cultures on substrates patterned with extracellular matrix (ECM) proteins have yielded much information in the past. However, although the culture of brain slices has many advantages over dissociated neuronal cultures, its feasibility on patterned substrates has not been demonstrated to date.In the present study, neuronal outgrowth from brain stem slices onto homogeneous control substrates, and onto laminin structures of grid- and line-shape was achieved. Cultures were evaluated by means of phase contrast microscopy, antibody staining, and patch-clamp measurements.Only patterns with line sizes of more than 4 μm yielded satisfactory neuronal outgrowth. The size of the nodes in the pattern influenced the nodal compliance of the spreading cells and the amount of unstructured overgrowth.Best grid patterns were 4 μm lines and 10 μm nodes, best line patterns were 4 μm lines and 20 μm nodes.On patterned substrates, average sodium and potassium currents were reduced by ∼50% compared to controls, whereas area-normalized ion-currents were in the same order of magnitude. This indicates that as a consequence of the pattern-enforced geometrical confinement, neurons tend to have a smaller surface. In addition, neurons on patterned substrates were rapidly covered with glial overgrowth. This was shown by antibody staining.

Keywords
Brain slices cultures; Cell migration; Micro-contact printing; Laminin; Patterned substrates; Electrophysiology
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Electrophysiological recordings of patterned rat brain stem slice neurons
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 23, Issue 15, August 2002, Pages 3123–3130
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us