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Acrylic acid grafting and collagen immobilization on poly(ethylene terephthalate) surfaces for adherence and growth of human bladder smooth muscle cells

Paper ID Volume ID Publish Year Pages File Format Full-Text
13665 889 2002 10 PDF Available
Title
Acrylic acid grafting and collagen immobilization on poly(ethylene terephthalate) surfaces for adherence and growth of human bladder smooth muscle cells
Abstract

In tissue engineering, degradable or non-degradable polymer matrices can act as cell-carrier-scaffolds. Cell adhesion and growth on these scaffolds can be promoted by immobilizing extracellular matrix proteins. Therefore, in this study, polymer poly(ethylene terephthalate) (PET) films were surface modified by graft polymerization of acrylic acid, to subsequently allow collagen (types I and III) immobilization and human smooth muscle cell expansion. The surfaces of PET were activated by plasma, followed by acrylic acid graft polymerization, resulting in covalently bound brushes, containing an average of either 0.22±0.1 or 5.93±0.87 μg/cm2 of poly(acrylic acid) (PAA). Subsequent electrostatic adsorption of collagen gave a surface concentration of 4.96 and 17.2 μg/cm2, respectively, as determined using radiolabelled 125I collagen. Both PET films grafted with 0.22 μg/cm2 of PAA with or without adsorbed collagen were apt for smooth muscle cell adhesion and proliferation. However, films grafted with 5.93 μg/cm2 were not. PAA-grafted PET films, onto which serum proteins of the culture medium adsorbed spontaneously, proved to be better matrices than films on which collagen has been immobilized. It, therefore, can be speculated that other serum proteins are more important than collagen for the human smooth muscle cell adhesion and growth on surface-modified polymer matrices.

Keywords
Poly(ethylene terephthalate); Plasma graft polymerization; Acrylic acid grafting; Collagen immobilization; Adherence and growth of human smooth muscle cells; Tissue engineering
First Page Preview
Acrylic acid grafting and collagen immobilization on poly(ethylene terephthalate) surfaces for adherence and growth of human bladder smooth muscle cells
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 23, Issue 15, August 2002, Pages 3149–3158
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering