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Initial interaction of rat bone marrow cells with non-coated and calcium phosphate coated titanium substrates

Paper ID Volume ID Publish Year Pages File Format Full-Text
13677 889 2002 9 PDF Available
Title
Initial interaction of rat bone marrow cells with non-coated and calcium phosphate coated titanium substrates
Abstract

Initial interactions of rat bone marrow (RBM) cells with smooth titanium, rough titanium or calcium phosphate coated substrates were tested. Cells were seeded onto the substrates, and attachment, integrin expression and spreading and morphology were studied.We found no difference in attachment of RBM cells to the different materials. We did find differences in the percentage of attached cells within a certain time between replicate runs of the experiments.RBM cells on all materials express α1, α3, α5, α6 and β1 subunits. Again there was a large difference in expression patterns on RBM cells in different runs. No difference was found in expression on the various materials. For α1, α5, α6 and β1, no difference was found in expression between attached and unattached cells. Expression of α3 was similar on attached and unattached cells during early culture. At the end of culture, α3 expression was downregulated for attached cells and not for unattached cells. This resulted in a higher expression of α3 for unattached cells compared to attached cells.Cells did spread on all materials, and reached a larger cell size on smooth titanium than on the rough materials. Morphology of the cells on the materials differed. On smooth titanium, cells usually showed a compact cell body with short cellular extensions. On the rough materials, cells often showed elongated shapes, with many thin cellular extensions.From this we conclude that the substrate surface characteristics of the materials we used do not influence attachment or integrin expression during the initial cell–material interactions. On the other hand, spreading behavior and cell morphology do depend on substrate surface characteristics.

Keywords
Rat bone marrow cells; Calcium phosphates; Titanium; Attachment; Spreading; Integrins
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Initial interaction of rat bone marrow cells with non-coated and calcium phosphate coated titanium substrates
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 23, Issue 15, August 2002, Pages 3269–3277
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us