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The role of silicon in osteoblast-like cell proliferation and apoptosis

Paper ID Volume ID Publish Year Pages File Format Full-Text
1368 82 2011 11 PDF Available
Title
The role of silicon in osteoblast-like cell proliferation and apoptosis
Abstract

The optimal concentration at which Si induces cell functions has not been fully elucidated. In the present study the effects of Si concentration (0–6 mM) on the biological functions of MG63 cells were investigated. Cell proliferation in the presence of 2 mM Si- and 4 mM Si-containing media progressively increased with culture time, whereas that of 6 mM Si treated MG63 cells was significantly (P < 0.05) reduced. The unusually high Si concentration (6 mM) induced a significant (P < 0.05) increase in the sub-G1 phase of cells from the original 3.60% up to 43.01% after culture for 12 h. In contrast, the other lower Si concentration treated MG63 cells in the sub-G1 phase were in the range 3–5% at all culture time points. 4 mM Si treated MG63 cells, but not 6 mM Si treated MG63 cells, showed remarkably enhanced collagen type I (COL I) gene expression and extracellular signal-regulated kinase (ERK) secretion, which were significantly (P < 0.05) higher than those in the control medium. The activation of ERK was also stimulated in MG63 cells by 4 mM Si. Cells cultured in the presence of 4 mM Si were found to have calcium matrix formation on day 7 that was 15-fold greater than that in the control medium. The results obtained in this study may be useful in designing calcium silicate-based materials with optimal biological properties.

Keywords
Calcium silicate; Cell viability; Cell proliferation; Apoptosis; Silicon
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The role of silicon in osteoblast-like cell proliferation and apoptosis
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Publisher
Database: Elsevier - ScienceDirect
Journal: Acta Biomaterialia - Volume 7, Issue 6, June 2011, Pages 2604–2614
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us