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Colonization of ion-modified polyethylene with vascular smooth muscle cells in vitro

Paper ID Volume ID Publish Year Pages File Format Full-Text
13701 892 2002 8 PDF Available
Title
Colonization of ion-modified polyethylene with vascular smooth muscle cells in vitro
Abstract

Polyethylene (PE) foils were implanted with 40 and 150 keV Ar+ ions to the fluences from 1×1013 to 1×1015 cm−2. Production of conjugated double bonds, characterizing degradation of the PE surface layer, was studied using UV–VIS spectroscopy. Wettability of the PE surface, determined by conventional goniometric techniques, was shown to be an increasing function of both ion energy and fluence. It was also increased after exposure of PE to serum-supplemented cell culture media. Cell culture experiments showed that the ion irradiation significantly increased the adherence of vascular smooth muscle cells (VSMC) and their subsequent growth on the PE surface. On day 1 after seeding, the number of initially adhered VSMC exhibited two maxima. On day 3 after seeding, these maxima disappeared, which was partially due to a significantly shorter doubling time of VSMC. On the other ion-modified samples, the doubling time did not differ significantly from that on the unmodified PE. Enzyme-linked immunosorbent assay revealed increased concentration of talin, a protein of focal adhesion plaques, and alpha-actin, a marker of VSMC differentiation, in cells on ion-implanted surfaces. It can be concluded that the ion irradiation supports the adhesion and differentiation of VSMC without excessive proliferation of these cells.

Keywords
Ion-implanted polyethylene; Wettability; Vascular smooth muscle cells; Adhesion; Proliferation; ELISA; Alpha-actin; Talin
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Colonization of ion-modified polyethylene with vascular smooth muscle cells in vitro
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 23, Issue 14, July 2002, Pages 2989–2996
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us