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In situ crosslinking of a biomimetic peptide-PEG hydrogel via thermally triggered activation of factor XIII

Paper ID Volume ID Publish Year Pages File Format Full-Text
13744 897 2002 8 PDF Available
Title
In situ crosslinking of a biomimetic peptide-PEG hydrogel via thermally triggered activation of factor XIII
Abstract

There is a medical need for robust, biocompatible hydrogels that can be rapidly crosslinked in situ through the use of gentle and non-toxic triggers, which could be used as a surgical adhesive, a bone-inductive material, or for drug and gene delivery. The complete gelation system described here includes calcium-loaded liposomes, hrFactor XIII, thrombin, and an enzymatic substrate based on a four-armed PEG in which each arm terminates with a 20mer peptide sequence derived from the γ-chain of fibrin. Controlled release of calcium ions for efficient hrFXIII activation was accomplished by thermal triggering of a tailored liposome phase transition at 37°C, which allowed the entire gelation system to be stored in aqueous solution at room temperature without premature gelation. When the system temperature was raised to 37°C (body temperature), the released calcium activates the hrFactor XIII, and gelation was observed to occur within 9 min. Rheological studies performed to quantitatively determine the storage modulus (G′) of the gel during oscillatory shear show that it behaves as a robust, elastic solid. Scanning electron microscopy studies revealed the hydrogel to have a very dense morphology overall, however spherical voids are observed in regions where calcium-loaded liposomes were entrapped during gelation.

Keywords
Liposome; Hydrogel; Peptide; Biomimetic; Poly(ethylene glycol); Enzymatic crosslinkingHuman recombinant factor XIII; hrFXIII; Scanning electron microscopy; SEM; Calcium-encapsulated interdigitation-fusion vesicles; Ca-IFVs; Poly(ethylene glycol); PEG
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In situ crosslinking of a biomimetic peptide-PEG hydrogel via thermally triggered activation of factor XIII
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 23, Issue 13, July 2002, Pages 2703–2710
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us