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In vitro degradation of chitosan by bacterial enzymes from rat cecal and colonic contents

Paper ID Volume ID Publish Year Pages File Format Full-Text
13751 897 2002 6 PDF Available
Title
In vitro degradation of chitosan by bacterial enzymes from rat cecal and colonic contents
Abstract

The degradative activities of extracellular and cell-associated portions of rat cecal and colonic enzymes, whose activities are comparable to that in the human colon, against five chitosan samples were characterized. The effects of the molecular weight (MW) and degree of deacetylation (DD) of chitosan on its susceptibility to degradation were investigated. In addition, the degradation function of rat bacterial enzymes was compared to that of a commercially available almond emulsin β-glucosidase that contains a chitinase. The results show that rat bacterial enzymes had the ability to degrade chitosan with extracellular enzymes exhibiting a more profound effect than did cell-associated enzymes. The reaction to bacterial enzymes degradation was dependent on both the MW and DD of the chitosan sample. Those samples with a lower MW and lower DD were more susceptible substrates. A similar degradation function of rat bacterial enzymes and of almond emulsin β-glucosidase on chitosan was revealed, which indicates that almond emulsin β-glucosidase might be able to be used as an in vitro enzyme system to predict the large intestinal degradation of chitosan.

Keywords
Chitosan; Rat cecal and colonic enzymes; Degradation; Molecular weight; Degree of deacetylation; Almond emulsin β-glucosidase
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In vitro degradation of chitosan by bacterial enzymes from rat cecal and colonic contents
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 23, Issue 13, July 2002, Pages 2761–2766
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us