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Optimization of diamine bridges in glutaraldehyde treated bioprosthetic aortic wall tissue

Paper ID Volume ID Publish Year Pages File Format Full-Text
13759 900 2002 5 PDF Available
Title
Optimization of diamine bridges in glutaraldehyde treated bioprosthetic aortic wall tissue
Abstract

Objective: Bioprosthetic calcification can be significantly mitigated by both increased concentrations of glutaraldehyde (GA) and the introduction of diamine (DA) bridges. The purpose of the present study was to evaluate whether an optimal effect of DA-enhanced fixation can be achieved by titration of dialdehyde and diamine concentrations.Methods: Porcine aortic roots were fixed at 0.05% GA (under-fixation) or 0.2% GA and 0.7% GA (commercial fixation). An interim step of DA treatment (l-Lysine; 0, 25, 50 or 100 mm; 37°C; 2 days) was followed by completion of the GA fixation (37°C; 5 days). Aortic wall coupons (12 mm) were punched out and implanted subcutaneously into seven-week old Long-Evans rats for 60 days. Calcium content was assessed by atomic absorption spectroscopy and histology.Results: Increasing the l-Lysine concentrations beyond 25 mm was essential to achieve the anti-calcific effect of DA-enhanced fixation. This effect was proportional to the GA concentrations applied. Compared to non-enhanced GA fixation (0 mm DA), calcification increased by 17.4% (p=0.2114) in 0.05% fixed tissue but decreased by 32.0% (p<0.0001) and 45.1% (p<0.0002) in 0.2% and 0.7% GA, respectively, when the DA concentration was 100 mm. Histologically the extent, but not the pattern of calcification, was affected.Conclusion: The calcium mitigating effect of diamine-treatment as an interim step of glutaraldehyde fixation is proportional to the GA concentration applied. Within commercial 0.7% GA fixation 100 mm DA has the potential to practically halve aortic wall calcification.

Keywords
Diamine bridges; Bioprosthetic calcification; Aortic wall tissue
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Optimization of diamine bridges in glutaraldehyde treated bioprosthetic aortic wall tissue
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 23, Issue 10, May 2002, Pages 2099–2103
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us