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Comparison of the antioxidant properties of HYAFF®-11p75, AQUACEL® and hyaluronan towards reactive oxygen species in vitro

Paper ID Volume ID Publish Year Pages File Format Full-Text
13777 900 2002 10 PDF Available
Title
Comparison of the antioxidant properties of HYAFF®-11p75, AQUACEL® and hyaluronan towards reactive oxygen species in vitro
Abstract

In chronic wounds, a number of host factors are released which perpetuate the inflammatory process, including polymorphonuclear leukocyte (PMN)-derived reactive oxygen species (ROS), such as superoxide radical (O2−) and hydroxyl radical (OH) species. The glycosaminoglycan, hyaluronan, has been shown to act as an antioxidant towards ROS, although the potential for biomaterials, such as HYAFF®-11p75 (the 75% benzyl ester of hyaluronan) and AQUACEL® (carboxymethylcellulose), to act in this manner has yet to be elucidated. This study compared the antioxidant properties of high and low molecular weight hyaluronan (HMWT HA and LMWT HA), HYAFF®-11p75, AQUACEL® and an AQUACEL®/hyaluronan composite (AQUACEL®/HA) against O2− and OH. The antioxidant capacities of each material were assessed by their ability to inhibit cytochrome C reduction by O2− fluxes, generated via the oxidation of hypoxanthine by xanthine oxidase, and their inhibition of 2-deoxy-d-ribose degradation by OH fluxes, generated by the reaction of hydrogen peroxide (H2O2) and iron (Fe2+).All materials studied possessed dose dependent antioxidant properties towards O2−, with HYAFF®-11p75 having the greatest antioxidant potential towards these species, followed by AQUACEL®, HMWT HA, AQUACEL®/HA and LMWT HA. Only HMWT HA exhibited dose dependent antioxidant properties towards OH at the fluxes examined.Gas chromatography/mass spectrometry analysis implied that ester bonds between the hyaluronan backbone and benzyl groups of HYAFF®-11p75 are highly susceptible to O2− hydrolysis, with the de-esterified benzyl alcohol being rapidly degraded in the presence of OH. This data supports the hypothesis that HYAFF®-11p75 has greater antioxidant capacity towards O2−, due to the esterified benzyl groups providing alternative sites for O2− attack other than the hyaluronan backbone of HYAFF®-11p75 itself and explains the inability of HYAFF®-11p75 to scavenge OH, due to benzyl alcohol degradation by OH.The antioxidant activities of these biomaterials, particularly HYAFF®-11p75 and AQUACEL®, towards O2− could be beneficial, as the scavenging of PMN-derived O2− may remove initial sources of O2− and further prevent the secondary formation of OH. These ROS are thought to be a primary causal factor for the extensive degradation and metabolic alterations observed in chronic wounds.

Keywords
DOR: 2-deoxy-d-ribose; ECM: Extracellular matrix; GAG: Glycosaminoglycans; H2O2: Hydrogen peroxide; HMWT HA: High molecular weight hyaluronan; LMWT HA: Low molecular weight hyaluronan; O2−: Superoxide radical; OH: Hydroxyl radical; PMN: Polymorphonuclear
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Comparison of the antioxidant properties of HYAFF®-11p75, AQUACEL® and hyaluronan towards reactive oxygen species in vitro
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 23, Issue 10, May 2002, Pages 2255–2264
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us