Structural alterations of adhesion mediating components in cells cultured on poly-β-hydroxy butyric acid
Polymers may serve as a biodegradable material in tissue engineering. To assess the biocompatibility of poly-β-hydroxy butyric acid (PHB), we studied the structural organization of cellular molecules involved in adhesion using osteoblastic and epithelial cell lines. On PHB, both cell lines revealed a rounded cell shape due to reduced spreading. The filamentous organization of the actin cytoskeleton was impaired. In double immunofluorescence analyses we demostrated that the colocalization of the fibronectin fibrils with the actin filaments was lost in cultures on PHB. Similarly, collagen II distribution was altered, whereas the organization of collagen I was not obviously affected. Further evidence for impaired structural organization was obtained for the β1-integrin receptor and vinculin which mediate the interaction of the cytoskeleton with the extracellular matrix. In confluent epithelial cells, the tight junction protein ZO-1 showed a larger lateral extension in the cell–cell contacts when cells were grown on PHB. Because structural organization of components which mediate cell–matrix and cell–cell adhesion controls cell physiology these parameters could be a sensitive indicator for the biocompatibility of implant materials.
Journal: Biomaterials - Volume 22, Issue 17, September 2001, Pages 2425–2434