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Substrate affects the initial attachment and subsequent behavior of human osteoblastic cells (Saos-2)

Paper ID Volume ID Publish Year Pages File Format Full-Text
13838 915 2001 9 PDF Available
Title
Substrate affects the initial attachment and subsequent behavior of human osteoblastic cells (Saos-2)
Abstract

Interaction between implant materials and bone cells contributes to the clinical success of dental implants. The object of this study was to investigate the initial attachment and subsequent behavior of human osteoblastic cells (Saos-2) to pure titanium (Ti), hydroxyapatite (HA), and glass. We, therefore, performed a time-course study for examining the area, attachment rate, distribution of focal adhesion kinase (FAK) vinculin, and actin, and the motility of Saos-2 cells on the materials. On Ti, cell area increased gradually, whereas on HA, cells spread quickly, but quitted spreading at 12 h after cell seeding. The number of cells on HA was greater than on the other materials. On Ti, the numbers of FAK- and vinculin-positive focal adhesions increased continuously. On HA, although the number of FAK-positive focal adhesions also increased continuously, the number of vinculin-positive focal adhesions decreased. Furthermore, actin staining showed that the cells on HA poorly formed stress fibers with weak polarity, whereas the cell on Ti possessed well-defined polarized stress fibers. On HA, cells started extension earlier than on Ti, motility was inactive, and the cells settled on the materials. These results suggest that the earlier settling of osteoblasts on HA might result in earlier osteogenesis on HA than other materials.

Keywords
Osteoblastic cell; FAK; Vinculin; Hydroxyapatite; Titanium
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Substrate affects the initial attachment and subsequent behavior of human osteoblastic cells (Saos-2)
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 22, Issue 16, 15 August 2001, Pages 2263–2271
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us