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Smooth muscle cell adhesion to tissue engineering scaffolds

Paper ID Volume ID Publish Year Pages File Format Full-Text
13888 940 2000 8 PDF Available
Title
Smooth muscle cell adhesion to tissue engineering scaffolds
Abstract

Synthetic polyesters of lactic and glycolic acid, and the extracellular matrix molecule collagen are among the most widely-utilized scaffolding materials in tissue engineering. However, the mechanism of cell adhesion to these tissue engineering scaffolds has not been extensively studied. In this paper, the mechanism of adhesion of smooth muscle cells to these materials was investigated. Vitronectin was found to be the predominant matrix protein adsorbed from serum-containing medium onto polyglycolic acid, poly(lactic co-glycolic) acid, and collagen two-dimensional films and three-dimensional scaffolds. Fibronectin adsorbed to both materials as well, although to a much lower density. Smooth muscle cell adhesion was mediated through specific integrin receptors interacting with these adsorbed proteins, as evidenced by both immunostaining and blocking studies. The receptors involved in adhesion included the αvβ5 to vitronectin, the α5β1 to fibronectin and the α2β1 to collagen I. Identification of the specific receptors used to adhere to these polymers clarifies why smooth muscle tissue development differs on these scaffolds, and may allow one to design tissue formation by controlling the surface chemistry of tissue engineering scaffolds.

Keywords
Polyglycolic acid; Vitronectin; Extracellular matrix; Collagen; Integrins
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Smooth muscle cell adhesion to tissue engineering scaffolds
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 21, Issue 20, October 2000, Pages 2025–2032
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us