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Expression of the CD69 activation antigen on lymphocytes of patients with hip prosthesis

Paper ID Volume ID Publish Year Pages File Format Full-Text
13892 940 2000 7 PDF Available
Title
Expression of the CD69 activation antigen on lymphocytes of patients with hip prosthesis
Abstract

The aim of the study was to evaluate the sensitization to metals in patients with Co–Cr hip prosthesis. Peripheral blood mononuclear cells (PBMC) were collected from 14 healthy donors and three groups of patients: 10 candidates for primary total joint replacements, 11 patients with well-fixed implant and 13 patients with aseptic loosening of the hip prosthesis. PBMCs were cultured with the metal ions employed for implant manufacturing and the expression of CD69 activation antigen on CD3/T lymphocytes was detected by flow cytometry. Chromium extract increased significantly the expression of CD3/CD69 phenotype in patients with loosening of hip prosthesis. The chromium-induced `activation index’ was higher in patients with loosening of hip prosthesis than in healthy donors and in pre-implant patients. The cobalt-stimulated PBMC of patients with either well-fixed or loosened prosthesis had an `activation index’ significantly higher than healthy donors. The activation index values were used to graduate the PBMC-response as `normal’ (⩾0.9 and <2), `low’ (<0.9) and `high’ (⩾2): an high-activation index was observed only in chromium-exposed PBMC of patients with prosthesis. Our data show that chromium released from orthopedic implants could be responsible for the lymphocyte sensitization and flow cytometry is an easy and reliable method for monitoring the hypersensitivity state in patients with metal prostheses. Activated lymphocytes in the peri-implant tissue are likely to elicit a localized immune response and contribute to maintain the inflammatory process evolving in the implant failure.

Keywords
Hip prosthesis; Metal corrosion; Activation antigen; Lymphocyte sensitization
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Expression of the CD69 activation antigen on lymphocytes of patients with hip prosthesis
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 21, Issue 20, October 2000, Pages 2059–2065
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us