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Intraseptal implantation of NGF-releasing microspheres promote the survival of axotomized cholinergic neurons

Paper ID Volume ID Publish Year Pages File Format Full-Text
13897 940 2000 5 PDF Available
Title
Intraseptal implantation of NGF-releasing microspheres promote the survival of axotomized cholinergic neurons
Abstract

Neurotrophic factors therapy requires their precise delivery to the targeted neuronal population. For this purpose, a wide range of strategies have been developed, and among them the stereotaxic implantation of biodegradable microparticles. To assess the in vivo activity of NGF-releasing PLGA microspheres, unloaded and NGF-loaded microparticles were implanted in the rat brain, near the septal cholinergic neurons, axotomized by an unilateral transection of the fornix-fimbria. Histological analysis at two and six weeks after implantation revealed a non-specific astro- and micro-glial reaction around the microspheres, identical for both unloaded and NGF-loaded microspheres. No neuronal toxicity was noticed, and healthy looking neurons were observed in contact with the microspheres. In the non-treated animals, the percentage of axotomized surviving neurons, when compared to the controlateral intact side, was 31±2 and 27±1% at two and six weeks, respectively. Unloaded microspheres caused no protective nor neurotoxic effects (40±9 and 39±6% of surviving cholinergic neurons at two and six weeks, respectively). In contrast, NGF-loaded microspheres showed a significant effect on the survival of axotomized cholinergic neurons at two and six weeks after implantation (66±9 and 61±5% when compared to the controlateral intact side, respectively). These results show that PLGA microparticles present no neurotoxicity and release sufficient amounts of bioactive NGF to significantly limit the lesion-induced disappearance of cholinergic neurons in the septum during at least six weeks. PLGA microparticles can be used in the future to administer neurotrophic factors in central nervous system disorders.

Keywords
Cholinergic neuron; Drug delivery; Microsphere; Nerve growth factor; Neuroprotection; Eurotrophic factor
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Intraseptal implantation of NGF-releasing microspheres promote the survival of axotomized cholinergic neurons
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 21, Issue 20, October 2000, Pages 2097–2101
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us