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Polyethylene particles of a ‘critical size’ are necessary for the induction of cytokines by macrophages in vitro

Paper ID Volume ID Publish Year Pages File Format Full-Text
13906 941 1998 6 PDF Available
Title
Polyethylene particles of a ‘critical size’ are necessary for the induction of cytokines by macrophages in vitro
Abstract

Particulate wear debris from total hip prosthetic components can stimulate macrophages to produce mediators of osteolysis which may cause aseptic implant loosening. This study evaluated the in vitro response of murine peritoneal macrophages to polyethylene particles of difinitive size distributions at varying volume doses. Ceridust 3615 polyethylene particles with a mean size of 0.21, 0.49, 4.3 and 7.2 μm and GUR1120 polyethylene resin with a mean size of 88 μm were co-cultured with C3H murine peritoneal macrophages at volume (μm)3 to cell number ratios of 100 : 1, 10 : 1, 1 : 1 and 0.1 : 1. The secretion of IL-6, IL-1β and TNF-α was determined by ELISA. Significantly elevated levels of TNF-α and IL-1β were determined at 100 : 1 ratios when the macrophages were challenged with particles with a mean size of 0.49, 4.3 and 7.2 μm, and at 10 : 1 ratios for particles with a mean size of 0.49 and 4.3 μm. IL-6 production was significantly elevated at 100 : 1 ratios for mean particle sizes of 0.49 and 4.3 μm. Particles outside this range produced considerably less cytokine suggesting that both the size and volume (or number) of polyethylene particles are critical factors in macrophage activation. Therefore particles in the phagocytosable size range of 0.3–10 μm appear to be the most biologically active.

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Polyethylene particles of a ‘critical size’ are necessary for the induction of cytokines by macrophages in vitro
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 19, Issue 24, December 1998, Pages 2297-2302
Authors
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us