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Evaluation of protein-modulated macrophage behavior on biomaterials: designing biomimetic materials for cellular engineering

Paper ID Volume ID Publish Year Pages File Format Full-Text
13917 974 1999 9 PDF Available
Title
Evaluation of protein-modulated macrophage behavior on biomaterials: designing biomimetic materials for cellular engineering
Abstract

Macrophage is a central cell type in directing host inflammatory and immune processes; hence, its response to biomaterials (i.e. adhesion and giant cell formation) has a direct impact on material biostability and biocompatibility. In this paper, several in vitro and in vivo techniques from previously published results and current investigations are highlighted and presented to demonstrate means of delineating a part of the complex molecular mechanisms involved in the interaction between biomaterials and macrophages. Complement component C3 was found critical in mediating the initial adhesion of human macrophages on medical-grade polyetherurethaneureas. From radioimmunoassay studies, the presence of a diphenolic antioxidant additive in polyetherurethaneureas increased the propensity for complement upregulation but did not affect adherent macrophage density. The subcutaneous cage-implant system was utilized to confirm the role of interleukin-4 in the fusion of adherent macrophages to form foreign body giant cells on polyurethanes in vivo. To probe the function-structural relationship of macrophage-active proteins, fibronectin was employed as a model in the formulation of synthetic oligopeptide mimetics. Peptides were grafted onto previously developed, non-cell adhesive polyethyleneglycol-based networks. The results indicate that grafted tripeptide RGD sequence supported higher adherent macrophage density than surfaces grafted with other peptides such as PHSRN and PRRARV sequences. However, the formation of foreign body giant cells on peptide-grafted networks was highly dependent on the relative orientation between PHSRN and RGD sequences located in a single peptide.

Keywords
Foreign body giant cells; Polyurethane; Polyethyleneglycol; Peptide; Inflammation
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Evaluation of protein-modulated macrophage behavior on biomaterials: designing biomimetic materials for cellular engineering
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 20, Issues 23–24, December 1999, Pages 2213–2221
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us