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Cellular recognition of synthetic peptide amphiphiles in self-assembled monolayer films

Paper ID Volume ID Publish Year Pages File Format Full-Text
13922 974 1999 15 PDF Available
Title
Cellular recognition of synthetic peptide amphiphiles in self-assembled monolayer films
Abstract

The incorporation of lipidated cell adhesion peptides into self-assembled structures such as films provides the opportunity to develop unique biomimetic materials with well-organized interfaces. Synthetic dialkyl tails have been linked to the amino-terminus, carboxyl-terminus, and both termini of the cell recognition sequence Arg–Gly–Asp (RGD) to produce amino-coupled, carboxyl-coupled, and looped RGD peptide amphiphiles. All three amphiphilic RGD versions self-assembled into fairly stable mixed monolayers that deposited well as Langmuir–Blodgett films on surfaces, except for films containing amino-coupled RGD amphiphiles at high peptide concentrations. FT-IR studies showed that amino-coupled RGD head groups formed the strongest lateral hydrogen bonds. Melanoma cells spread on looped RGD amphiphiles in a concentration-dependent manner, spread indiscriminately on carboxyl-coupled RGD amphiphiles, and did not spread on amino-coupled RGD amphiphiles. Looped RGD amphiphiles promoted the adhesion, spreading, and cytoskeletal reorganization of melanoma and endothelial cells while control looped Arg–Gly–Glu (RGE) amphiphiles inhibited them. Antibody inhibition of the integrin receptor α3β1 blocked melanoma cell adhesion to looped RGD amphiphiles. These results confirm that novel biomolecular materials containing synthetic peptide amphiphiles have the potential to control cellular behavior in a specific manner.

Keywords
Biomimetic materials; Cell adhesion; Cell spreading; Langmuir–Blodgett films; Peptide amphiphiles; RGD
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Cellular recognition of synthetic peptide amphiphiles in self-assembled monolayer films
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 20, Issues 23–24, December 1999, Pages 2265–2279
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us