fulltext.study @t Gmail

Retinal pigment epithelial cell function on substrates with chemically micropatterned surfaces

Paper ID Volume ID Publish Year Pages File Format Full-Text
13930 974 1999 11 PDF Available
Title
Retinal pigment epithelial cell function on substrates with chemically micropatterned surfaces
Abstract

Model substrates with desired chemical micropatterns were fabricated using a microcontact printing technique. The substrate surfaces contained organized arrays of circular glass domains with a diameter of either 10 or 50 μm surrounded and separated by regions modified with octadecyltrichlorosilane (OTS) self-assembled monolayers (SAMs). The effects of surface patterning on in vitro cell attachment, proliferation, morphology, and cytoskeletal organization were evaluated using a human retinal pigment epithelium (RPE) cell line. Both micropatterns affected initial RPE cell attachment, limited cell spreading, and promoted the characteristic cuboidal cell morphology throughout the culture period. In contrast, RPE cells on plain glass control were elongated and appeared fibroblast-like prior to confluence. In addition, cells seeded at 30 000 cell/cm2 on the patterned surfaces maintained a normal pattern of actin and cytokeratin expression, and formed confluent monolayers within 4 days of culture. The cell density increased about 30-fold on both micropatterns by day 7. These results show that it is feasible to control RPE cell shape and expression of differentiated phenotype using micropatterned surfaces.

Keywords
Micropatterned surface; Retinal pigment epithelium; Cell function; Microcontact printing; Self-assembled monolayer
First Page Preview
Retinal pigment epithelial cell function on substrates with chemically micropatterned surfaces
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 20, Issues 23–24, December 1999, Pages 2351–2361
Authors
, , , , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering