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An assessment of the strength of NG108-15 cell adhesion to chemically modified surfaces

Paper ID Volume ID Publish Year Pages File Format Full-Text
13935 974 1999 9 PDF Available
Title
An assessment of the strength of NG108-15 cell adhesion to chemically modified surfaces
Abstract

The strength of adhesion of NG108-15 cells to glass substrates modified with adsorbed proteins (laminin and poly-ornithine) or modified with covalently bound peptides (tri-ornithine and Tyr–Ile–Gly–Ser–Arg) was quantitatively assessed, by determining the shear stresses necessary to denude the cells from substrates using a spinning disk device. The shear stresses required to detach NG108-15 cells from glass modified with either adsorbed poly-ornithine or with both poly-ornithine and laminin were significantly (P<0.05) higher than the shear stresses required to detach the cells from plain glass substrates. Covalent surface modifications resulted in higher strengths of NG108-15 adhesion than were exhibited on surfaces modified with adsorbed proteins. NG108-15 cell adhesion strength was maximal on surfaces covalently modified with only amine groups (without any peptides or proteins). These results indicate that general (i.e., not necessarily receptor-specific) surface modification strategies, which increase the net surface charge of a substrate, will elicit strong adhesion of NG108-15 cells.

Keywords
YIGSR; Poly-ornithine; Covalent surface modification; Neural; Cell attachment; NG108-15
First Page Preview
An assessment of the strength of NG108-15 cell adhesion to chemically modified surfaces
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 20, Issues 23–24, December 1999, Pages 2417–2425
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering