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In vivo cell recruitment, cytokine release and chemiluminescence response at gold, and thiol functionalized surfaces

Paper ID Volume ID Publish Year Pages File Format Full-Text
13943 977 1999 15 PDF Available
Title
In vivo cell recruitment, cytokine release and chemiluminescence response at gold, and thiol functionalized surfaces
Abstract

Hydroxylated and methylated surfaces were prepared by the self-assembled monolayer technique (SAM) of alkane thiols on gold. The surfaces were used to evaluate the influence of implant surface chemistry on protein deposition and inflammatory cell response. Implants were inserted subcutaneously in the rat for 3 and 24 h. The surface chemical properties influenced the in vitro rat plasma protein adsorption (ellipsometry/antibody) with few exceptions (albumin not found and fibrinogen always found). The number of recruited cells and their distribution (DNA from implant versus from exudate) was influenced by the different chemistries at 24 h, but not at 3 h. HIS48+, ED1+, ED2+ and small numbers of CD5+ cells were present in the exudate at both time periods (flow cytometry). The cellular oxidative metabolism was low, although cells on –OH surfaces responded with the highest phorbol ester-stimulated chemiluminescence (CL)/DNA. The levels of cytokines IL-1α, IL-1β and TNFα (ELISA) were not influenced by material surface chemistry. Sham operated sites had a higher cytokine concentration/DNA compared with exudates from an implant milieu. The results of this study show that surface chemical functionalization modifies specific events in the inflammatory response around implants in soft tissues.

Keywords
Biocompatibility; Chemiluminescence; Interleukin-1; Protein adsorption; Surface modification; Tumour necrosis factor alpha
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In vivo cell recruitment, cytokine release and chemiluminescence response at gold, and thiol functionalized surfaces
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 20, Issue 22, November 1999, Pages 2123–2137
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us