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The effect of alginate and hyaluronate on the viability and function of immunoisolated neonatal rat islets

Paper ID Volume ID Publish Year Pages File Format Full-Text
13947 977 1999 7 PDF Available
Title
The effect of alginate and hyaluronate on the viability and function of immunoisolated neonatal rat islets
Abstract

Recent observations suggest that the extracellular matrix plays a role as regards the viability and morphological integrity in long-term culture of Langerhans islets. For the present study we encapsulated neonatal rat islets without extracellular matrix (WEM), with alginate solution (AL) and with hyaluronate solution (HY) into cuprophane hollow fibers. Function was tested after week 1 and 5. The insulin release of WEM encapsulated islets decreased significantly during the culture period. In contrast to this, AL and HY embedded islets had stable secretion values throughout the whole cultivation. Histological examination confirmed that viability of HY and of AL embedded islets differed significantly from that of WEM encapsulated islets. Furthermore, HY seems to be a more advantageous environment to immunoisolated islets than AL. Both the insulin secretion values and the viability of HY embedded islets were higher than of AL embedded islets. We conclude that an extracellular matrix is important for immunoisolated islets, to maintain their function and morphological integrity and that HY is especially suitable for this application.

Keywords
Pancreatic islets; Bioartificial pancreas; Encapsulation; Alginate; Hyaluronic acid; Islet viability
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The effect of alginate and hyaluronate on the viability and function of immunoisolated neonatal rat islets
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 20, Issue 22, November 1999, Pages 2161–2167
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us