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Guided tissue fabrication from periosteum using preformed biodegradable polymer scaffolds

Paper ID Volume ID Publish Year Pages File Format Full-Text
13955 982 2007 12 PDF Available
Title
Guided tissue fabrication from periosteum using preformed biodegradable polymer scaffolds
Abstract

A successful tissue engineering method for bone replacement would imitate natural bone graft by providing the essential elements for new bone formation using synthetic scaffolds, osteogenic cell populations, and bone induction factors. This is a study of the suitability of various formulations of poly(dl-lactic-co-glycolic acid) (PLGA) foams to provide a tissue conducting scaffold in an ovine model for bone flap fabrication. Three formulations were used of different copolymer ratio and molecular weight. Porous wafers of PLGA were stacked into rectangular chambers (volume 4 cm3) enclosed on five sides. Some chambers also contained autologous morcellized bone graft (MBG). The chambers were inserted with the open face adjacent to the cambium layer of the periosteum in rib beds of seven sheep and harvested after 8 weeks in vivo. Gross and histologic examination of the resulting tissue specimens demonstrated molded units of vascularized tissue generally conforming to the shape of the chambers and firmly attached to the periosteum. Polymer degradation appeared to occur by varying degrees based on polymer formulation. New bone formation was observed only in areas containing MBG. There was no evidence of significant inflammatory reaction or local tissue damage at 8 weeks. We conclude that a PLGA foam scaffold is (1) an efficient conductor of new tissue growth but not osteoinductive, (2) contributes to the shape of molded tissue, and (3) biocompatible when used in this model. Further studies are warranted to develop practical methods to deliver bone induction factors to the system to promote osseous tissue generation throughout the synthetic scaffold.

Keywords
Tissue fabrication; Biodegradable polymer scaffolds
First Page Preview
Guided tissue fabrication from periosteum using preformed biodegradable polymer scaffolds
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 20, Issue 21, November 1999, Pages 2007–2018
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering