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Serum albumin–alginate coated beads: mechanical properties and stability

Paper ID Volume ID Publish Year Pages File Format Full-Text
13962 982 1999 16 PDF Available
Title
Serum albumin–alginate coated beads: mechanical properties and stability
Abstract

According to a previously described method, alginate beads were prepared from a Na–alginate solution containing propylene glycol alginate (PGA) and human serum albumin (HSA). The solution was added dropwise to a CaCl2 solution. The beads were treated with NaOH, which started the formation of amide bonds between HSA and PGA at the periphery, giving a membrane. Batches of beads with increasingly thick membranes were prepared using growing concentrations of NaOH, and studied with a texture analyser. When raising NaOH concentration, the rupture strength progressively increased, and the resistance strength to a deformation of 50% of total height also increased before slightly decreasing for the highest NaOH concentration. Variations of bead elasticity were also observed. When the beads were prepared with saline reducing gelation time from 10 to 5 min, and reaction time from 15 to 5 min, mechanical properties varied more progressively with the NaOH concentration, while the results became more reproducible. A series of assays conducted with 0.01 m NaOH confirmed the importance of using a short gelation time, and saline rather than water. Stability assays were also performed. The results were compared to those of alginate-polylysine coated beads and showed the interest of the transacylation method.

Keywords
Coated alginate beads; Propylene glycol alginate; Serum albumin; Mechanical properties; Texture analyser
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 20, Issue 21, November 1999, Pages 2069–2084
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us