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Basic thermoanalytical studies of insoluble collagen matrices

Paper ID Volume ID Publish Year Pages File Format Full-Text
13985 991 1996 6 PDF Available
Title
Basic thermoanalytical studies of insoluble collagen matrices
Abstract

Insoluble collagen has been utilized as a base material for parenteral drug carrier systems. Information on its physicochemical properties was obtained by focussing on thermoanalytical methods. On the way from the raw material to the matrices, the acidic aqueous dispersion represents an important intermediate state. DSC and FTIR revealed its complete denaturation at 43 °C. Dense homogeneous collagen matrices were prepared by air-drying at 25 °C and became denatured at 103.5 °C, far above normal storage temperatures. Dielectrical Thermal Analysis demonstrated transitions in the dielectrical storage and loss moduli, reflecting the dissipation of electrical energy and increased molecular mobility caused by collapse of the triple helical structure. Cross-linking of the collagen dispersion with glutaraldehyde induced no alteration in the thermoanalytical properties of dry matrices. However, in the swollen state, after incubation of the devices in phosphate buffer the transition temperature increased from 50 to 70 °C as cross-linking was intensified. This indicated stronger interactions between the collagen fibre structures. Dissolution tests with cytochrome c-loaded matrices showed that higher amounts of the model protein were trapped inside the matrices as more glutaraldehyde was added.

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Basic thermoanalytical studies of insoluble collagen matrices
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 17, Issue 23, 1996, Pages 2289-2294
Authors
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering