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Effect of low-intensity ultrasound upon biofilm structure from confocal scanning laser microscopy observation

Paper ID Volume ID Publish Year Pages File Format Full-Text
14037 1069 1980 6 PDF Available
Title
Effect of low-intensity ultrasound upon biofilm structure from confocal scanning laser microscopy observation
Abstract

Ultrasonic irradiation at 500kHz and 10 mW cm−2 of a 24 h old biofilm of P. aeruginosa enhanced the killing of bacteria by gentamicin. To determine whether this bioacoustic effect was caused by ultrasonic-induced changes in the biofilm morphology (biofilm breakup or disruption), the biofilms were examined by confocal scanning laser microscopy (CSLM). Such disruption would be undesirable in the possible ultrasonic treatment of implant infections. The CSLM results showed that the biofilm is a partial monolayer of cells with occasional aggregates of cells, non-cellular materials and extracellular spaces. The aggregates contained large amounts of exopolysaccharide. The structure of biofilm was not changed when the biofilm was exposed to continuous ultrasound at 500 k Hz and 10 mW cm−2, the same irradiation parameters that increased cell killing by nearly two orders of magnitude. The observation that low-intensity ultrasound does not disrupt biofilm or disperse the bacteria has significance in the possible use of ultrasound to enhance the action of antibiotics against biofilms.

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Effect of low-intensity ultrasound upon biofilm structure from confocal scanning laser microscopy observation
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomaterials - Volume 17, Issue 20, October 1996, Pages 1975-1980
Authors
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us