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Cost effective method for construction of high quality cDNA libraries

Paper ID Volume ID Publish Year Pages File Format Full-Text
14050 1070 2007 3 PDF Available
Title
Cost effective method for construction of high quality cDNA libraries
Abstract

A PCR-based method for the synthesis of high quality cDNA is described. This method combines the reverse transcriptase template switching with the assisted recombination cloning. It enables 5′ end enrichment, cDNA synthesis from limiting starting material, elimination of digestion and subcloning steps and substantial time and cost savings.

Keywords
cDNA library; Recombination; Cloning; Reverse transcription
First Page Preview
Cost effective method for construction of high quality cDNA libraries
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomolecular Engineering - Volume 24, Issue 4, October 2007, Pages 419–421
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering