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An AFM investigation of oligonucleotides anchored on unoxidized crystalline silicon surfaces

Paper ID Volume ID Publish Year Pages File Format Full-Text
14060 1072 2007 6 PDF Available
Title
An AFM investigation of oligonucleotides anchored on unoxidized crystalline silicon surfaces
Abstract

Carboxylic terminated monolayers have been covalently attached on phosphorous doped crystalline (1 0 0) silicon surfaces using a cathodic electro grafting technique. The functionalization concentration and efficiency have been evaluated with different techniques. In particular, topographic images, performed with an atomic force microscope, were used to optimize the protocol in order to obtain a surface whose characteristics of uniformity and reproducibility are ideal for a bio-electronic device. Phase lag images of the functionalized surfaces were also performed, and show non-topographic structures that have been interpreted as areas of different molecule self-orientation.Poly-thymine oligonucleotides have been anchored on such a surface to form a nano-biosensing device capable to react selectively with a specific target molecule, a poly-adenine oligonucleotide. AFM images of high density (∼3 × 1012 mol/cm2) single strand and double strand covered samples show toroidal shaped structures formed by the self-assembly of the oligonucleotides on the silicon surface.

Keywords
Oligonucleotides; Silicon surface; AFM topography; AFM Phase Lag
First Page Preview
An AFM investigation of oligonucleotides anchored on unoxidized crystalline silicon surfaces
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomolecular Engineering - Volume 24, Issue 1, February 2007, Pages 53–58
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering