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Atomic tritium as an instrument for study of protein behavior at the air–water interface

Paper ID Volume ID Publish Year Pages File Format Full-Text
14072 1072 2007 5 PDF Available
Title
Atomic tritium as an instrument for study of protein behavior at the air–water interface
Abstract

Atomic tritium was successfully applied as an instrument for study of protein behavior at the air–water interface. Samples of lysozyme solution in 20 mM phosphate buffer (pH 7.0) with concentration of 2 mg/ml incubated at the room temperature for 1 h were exposed to bombardment with tritium atoms generated on hot tungsten wire in special vacuum device. This procedure resulted in substitution of hydrogen atoms by radioactive tritium in the thin surface layer of studied preparations. Analysis of experimental data on intramolecular radioactivity distribution in lysozyme and computer simulation of tritium bombardment allowed us to suggest two equally probable opposite orientations of lysozyme molecule in the adsorption layer at the air–water interface.

Keywords
Atomic tritium; Adsorption; Adsorption layers; Air–water interface; Protein structure; Lysozyme
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomolecular Engineering - Volume 24, Issue 1, February 2007, Pages 125–129
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us