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The effect of ethylene oxide, glow discharge and electron beam on the surface characteristics of poly(l-lactide-co-caprolactone) and the corresponding cellular response of adipose stem cells

Paper ID Volume ID Publish Year Pages File Format Full-Text
1408 83 2010 6 PDF Available
Title
The effect of ethylene oxide, glow discharge and electron beam on the surface characteristics of poly(l-lactide-co-caprolactone) and the corresponding cellular response of adipose stem cells
Abstract

Bioabsorbable polymers are increasingly being used in tissue engineering strategies. Despite the knowledge that some sterilization techniques may affect the physical properties of these polymers, this aspect is often overlooked. We speculate that the type of sterilization method used may influence cellular responses by altering the surface characteristics. We cultured adipose stem cells on bioabsorbable poly(l-lactide-co-caprolactone) (PLCL) sheets, sterilized using either ethylene oxide (EO), argon glow discharge (aGD) or electron beam (e-beam). Significantly higher values for surface roughness in the order EO > aGD > e-beam and significant differences in contact angles (EO > e-beam > aGD) and surface energies (aGD > e-beam > EO) were observed. Increased cell attachment and proliferation rates were observed with lower contact angles. The alkaline phosphatase activity was significantly higher for the ethylene oxide sterilized PLCL sheet. In conclusion, the type of sterilization for bioabsorbable polymers should be considered in the design of new scaffolds, since it might affect, or can be used to enhance, the outcome of the tissue engineered construct.

Keywords
Stem cell; Biodegradation; Sterilization; Bone tissue engineering; Polylactic acid
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The effect of ethylene oxide, glow discharge and electron beam on the surface characteristics of poly(l-lactide-co-caprolactone) and the corresponding cellular response of adipose stem cells
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Publisher
Database: Elsevier - ScienceDirect
Journal: Acta Biomaterialia - Volume 6, Issue 6, June 2010, Pages 2060–2065
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us