fulltext.study @t Gmail

Effect of different growth factors on human osteoblasts activities: A possible application in bone regeneration for tissue engineering

Paper ID Volume ID Publish Year Pages File Format Full-Text
14082 1144 2007 6 PDF Available
Title
Effect of different growth factors on human osteoblasts activities: A possible application in bone regeneration for tissue engineering
Abstract

Cultured human primary osteoblasts reproduce the phenotypic differentiation and maturation of cells in vivo. We have investigated the influence of three isoforms of transforming growth factor beta (TGF-β1, TGF-β2 and TGF-β3), three fibroblast growth factors (FGF-2, FGF-4 and FGF-6) and the active metabolite of Vitamin D [1,25-(OH)2D3] on proliferation, alkaline phosphatase activity and mineralization of human osteoblasts during a period of 24 days of culture. TGF-β isoforms and three FGFs examined have been proved to be inducers of osteoblasts proliferation (higher extent for TGF-β and FGF-2) and inhibitors of alkaline phosphatase activity and osteoblasts mineralization. Combination of these growth factors with the active form of Vitamin D induced osteodifferentiation. In fact Vitamin D showed an additive effect on alkaline phosphatase activity and calcium content, induced by FGF-2 and TGF-β in human osteoblast. These results highlight the potential of proliferating cytokines’ combination with mineralizing agents for in vitro bone growth induction in bone tissue engineering.

Keywords
Human primary osteoblast; Growth factors; Active Vitamin D; Proliferation; Alkaline phosphatase; Mineralization
First Page Preview
Effect of different growth factors on human osteoblasts activities: A possible application in bone regeneration for tissue engineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomolecular Engineering - Volume 24, Issue 6, December 2007, Pages 613–618
Authors
, , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us