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Overexpression of GLT1 in fps1ΔgpdΔ mutant for optimum ethanol formation by Saccharomyces cerevisiae

Paper ID Volume ID Publish Year Pages File Format Full-Text
14086 1144 2007 5 PDF Available
Title
Overexpression of GLT1 in fps1ΔgpdΔ mutant for optimum ethanol formation by Saccharomyces cerevisiae
Abstract

Glycerol is the main byproduct produced under anaerobic ethanol fermentations by Saccharomyces cerevisiae and consumes a considerable amount of substrate. To verify the metabolic phenotype predications for increasing ethanol formation, two engineered S. cerevisiae KAM-14, KAM-15 strains were constructed for possible redirection of glycerol carbon flux into ethanol by overexpression of GLT1 in the fps1ΔgpdΔ mutant. The engineered strains KAM-14 and KAM-15 compared to the control strain KAM-2, produced 12.24% and 10.42% higher ethanol, 39.72% and 31.03% lower glycerol yield during anaerobic batch fermentations, respectively. The maximum specific growth rates of KAM-14 and KAM-15 were found to be relatively lower than that of KAM-2 during the exponential growth phase. In the meantime, the biomass concentrations of both KAM-14 and KAM-15 were similar to KAM-2. Acetate and pyruvate concentrations of KAM-14 and KAM-15 were greatly decreased comparing to those of KAM-2, respectively. These experimental results approved the metabolic pathway strategies to improve ethanol formation.

Keywords
Saccharomyces cerevisiae; Metabolic phenotype; Redox balance; Ethanol; Glycerol; Glutamate
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Overexpression of GLT1 in fps1ΔgpdΔ mutant for optimum ethanol formation by Saccharomyces cerevisiae
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomolecular Engineering - Volume 24, Issue 6, December 2007, Pages 638–642
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us