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Polyclonal antibodies mediated immobilization of a peroxidase from ammonium sulphate fractionated bitter gourd (Momordica charantia) proteins

Paper ID Volume ID Publish Year Pages File Format Full-Text
14095 1147 2007 8 PDF Available
Title
Polyclonal antibodies mediated immobilization of a peroxidase from ammonium sulphate fractionated bitter gourd (Momordica charantia) proteins
Abstract

Polyclonal antibody bound Sepharose 4B support has been exploited for the immobilization of bitter gourd peroxidase directly from ammonium sulphate precipitated proteins. Immunoaffinity immobilized bitter gourd peroxidase exhibited high yield of immobilization. IgG-Sepharose 4B bound bitter gourd peroxidase showed a higher stability against heat, chaotropic agents (urea and guanidinium chloride), detergents (cetyl trimethyl ammonium bromide and Surf Excel), proteolytic enzyme (trypsin) and water-miscible organic solvents (propanol, THF and dioxane). The activity of immobilized bitter gourd peroxidase was significantly enhanced in the presence of cetyl trimethyl ammonium bromide and after treatment with trypsin as compared to soluble enzyme.

Keywords
BGP, bitter gourd peroxidase; S-BGP, soluble bitter gourd peroxidase; I-BGP, immobilized bitter gourd peroxidase; HRP, horseradish peroxidase; THF, tetrahydrofuran; DEAE, diethyl aminoethyl; PAGE, polyacrylamide gel electrophoresis; SDS, sodium dodecyl su
First Page Preview
Polyclonal antibodies mediated immobilization of a peroxidase from ammonium sulphate fractionated bitter gourd (Momordica charantia) proteins
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomolecular Engineering - Volume 24, Issue 2, June 2007, Pages 223–230
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering