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An improved PCR-based amplification of unknown homologous DNA sequences

Paper ID Volume ID Publish Year Pages File Format Full-Text
14110 1148 2006 4 PDF Available
Title
An improved PCR-based amplification of unknown homologous DNA sequences
Abstract

The PCR primers used for cloning of evolutionary conserved genes or homologous DNA sequences are usually guessmer oligonucleotides. We introduce a simple way using Pfu polymerase to overcome possible PCR amplification failure because of 3′-end mismatches of guessed primers with the target DNA.

Keywords
PCR; Cloning; Mismatched primer; Homologous DNA
First Page Preview
An improved PCR-based amplification of unknown homologous DNA sequences
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomolecular Engineering - Volume 23, Issue 4, September 2006, Pages 209–212
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering