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Liquid-based hybridization assay with real-time detection in miniaturized array platforms

Paper ID Volume ID Publish Year Pages File Format Full-Text
14114 1150 2006 6 PDF Available
Title
Liquid-based hybridization assay with real-time detection in miniaturized array platforms
Abstract

An assay for the fluorescent detection of short oligonucleotide probe hybridization in miniaturized high-density array platforms is presented. It combines hybridization in solution with real-time fluorescent detection, which involves measurement of fluorescence increase by means of an induced fluorescence resonance energy transfer. The feasibility of this approach using DNA or RNA as a target, and short DNA- as well as LNA (locked nucleic acid)-modified oligonucleotides as probes is shown. The presented approach could potentially contribute to a significant increase in the throughput of large-scale genomic applications, such as oligofingerprinting and genotyping, and also reduce material consumption.

Keywords
Hybridization; iFRET; Locked nucleic acids; Oligonucleotide fingerprinting
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Liquid-based hybridization assay with real-time detection in miniaturized array platforms
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomolecular Engineering - Volume 23, Issue 1, March 2006, Pages 35–40
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us