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Targeted cDNA differential display (TcDD)

Paper ID Volume ID Publish Year Pages File Format Full-Text
14115 1150 2006 14 PDF Available
Title
Targeted cDNA differential display (TcDD)
Abstract

Targeted cDNA differential display (TcDD) was developed to study expression of a different selected gene families especially those at low copy numbers per cell. This method is an adaptation of our previously described targeted genomic differential display method (TGDD). In TcDD, the expression of genes containing target sequences such as CAG repeating sequences or genes encoding for zinc-finger binding proteins were followed in an experimental rat model with salt-induced hypertension. DNA sequencing experiments demonstrated that the effectiveness of targeting was greater than 99%.

Keywords
Differential display; cDNA; Gene expression; Gene families; Repeats
First Page Preview
Targeted cDNA differential display (TcDD)
Publisher
Database: Elsevier - ScienceDirect
Journal: Biomolecular Engineering - Volume 23, Issue 1, March 2006, Pages 41–54
Authors
, , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering