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Production of penicillin acylase by a recombinant Escherichia coli using cheese whey as substrate and inducer

Paper ID Volume ID Publish Year Pages File Format Full-Text
14164 1178 2006 7 PDF Available
Title
Production of penicillin acylase by a recombinant Escherichia coli using cheese whey as substrate and inducer
Abstract

Cheese whey (CW) is the major subproduct from cheese manufacturing and it is considered as a waste pollutant since its high content of lactose. In this work a fermentation process for the production of penicillin acylase (PA) by a recombinant Escherichia coli and using CW as unique carbon source and inducer was developed. A design factorial 32 was used to evaluate the influence of independent variables (dissolved oxygen and CW concentration) on the ability of E. coli W3110/pPA102 to produce PA. Maximum specific PA activity of 781 U g−1 was attained at 5 g L−1 of CW and 3% dissolved oxygen. The results showed that CW can be used successfully as unique carbon source and inducer for the production of recombinant proteins using constructions driven by the lac promoter and this way reducing the discharges of that pollutant to the environment.

Keywords
Batch processing; Growth kinetics; Lactose; Recombinant DNA; Protein
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Production of penicillin acylase by a recombinant Escherichia coli using cheese whey as substrate and inducer
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomolecular Engineering - Volume 23, Issue 6, December 2006, Pages 299–305
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us