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Protein engineering of formate dehydrogenase

Paper ID Volume ID Publish Year Pages File Format Full-Text
14179 1180 2006 22 PDF Available
Title
Protein engineering of formate dehydrogenase
Abstract

NAD+-dependent formate dehydrogenase (FDH, EC 1.2.1.2) is one of the best enzymes for the purpose of NADH regeneration in dehydrogenase-based synthesis of optically active compounds. Low operational stability and high production cost of native FDHs limit their application in commercial production of chiral compounds. The review summarizes the results on engineering of bacterial and yeast FDHs aimed at improving their chemical and thermal stability, catalytic activity, switch in coenzyme specificity from NAD+ to NADP+ and overexpression in Escherichia coli cells.

Keywords
Formate dehydrogenase; Protein engineering; Pseudomonas sp.101; Candida boidinii; Stability; Mutagenesis
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Protein engineering of formate dehydrogenase
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biomolecular Engineering - Volume 23, Issues 2–3, June 2006, Pages 89–110
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us