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Sucrose synthase: A unique glycosyltransferase for biocatalytic glycosylation process development

Paper ID Volume ID Publish Year Pages File Format Full-Text
14185 1186 2016 24 PDF Available
Title
Sucrose synthase: A unique glycosyltransferase for biocatalytic glycosylation process development
Abstract

•Application of sucrose synthase in biocatalytic glycosylation processes•Biochemical characterization, structural features and mechanistic insights•Biocatalyst production and impact of serine phosphorylation•Biocatalytic production of NDP-sugars and disaccharides•NDP-sugar regeneration for glycosyltransferase cascade reactions

Sucrose synthase (SuSy, EC 2.4.1.13) is a glycosyltransferase (GT) long known from plants and more recently discovered in bacteria. The enzyme catalyzes the reversible transfer of a glucosyl moiety between fructose and a nucleoside diphosphate (NDP) (sucrose + NDP ↔ NDP-glucose + fructose). The equilibrium for sucrose conversion is pH dependent, and pH values between 5.5 and 7.5 promote NDP-glucose formation. The conversion of a bulk chemical to high-priced NDP-glucose in a one-step reaction provides the key aspect for industrial interest. NDP-sugars are important as such and as key intermediates for glycosylation reactions by highly selective Leloir GTs. SuSy has gained renewed interest as industrially attractive biocatalyst, due to substantial scientific progresses achieved in the last few years. These include biochemical characterization of bacterial SuSys, overproduction of recombinant SuSys, structural information useful for design of tailor-made catalysts, and development of one-pot SuSy-GT cascade reactions for production of several relevant glycosides. These advances could pave the way for the application of Leloir GTs to be used in cost-effective processes. This review provides a framework for application requirements, focusing on catalytic properties, heterologous enzyme production and reaction engineering. The potential of SuSy biocatalysis will be presented based on various biotechnological applications: NDP-sugar synthesis; sucrose analog synthesis; glycoside synthesis by SuSy-GT cascade reactions.

Keywords
GT, glycosyltransferase; NDP, nucleoside diphosphate; NMP, nucleoside monophosphate; NTP, nucleoside triphosphate; SuSy, sucrose synthaseGlycobiotechnology; Glycosyltransferase; Sucrose synthase; Glycosylation; Glycosides; Nucleotide-activated sugars; Nuc
First Page Preview
Sucrose synthase: A unique glycosyltransferase for biocatalytic glycosylation process development
Publisher
Database: Elsevier - ScienceDirect
Journal: Biotechnology Advances - Volume 34, Issue 2, March–April 2016, Pages 88–111
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering