fulltext.study @t Gmail

Recombinant CBM-fusion technology — Applications overview

Paper ID Volume ID Publish Year Pages File Format Full-Text
14220 1191 2015 12 PDF Available
Title
Recombinant CBM-fusion technology — Applications overview
Abstract

Carbohydrate-binding modules (CBMs) are small components of several enzymes, which present an independent fold and function, and specific carbohydrate-binding activity. Their major function is to bind the enzyme to the substrate enhancing its catalytic activity, especially in the case of insoluble substrates. The immense diversity of CBMs, together with their unique properties, has long raised their attention for many biotechnological applications. Recombinant DNA technology has been used for cloning and characterizing new CBMs. In addition, it has been employed to improve the purity and availability of many CBMs, but mainly, to construct bi-functional CBM-fused proteins for specific applications. This review presents a comprehensive summary of the uses of CBMs recombinantly produced from heterologous organisms, or by the original host, along with the latest advances. Emphasis is given particularly to the applications of recombinant CBM-fusions in: (a) modification of fibers, (b) production, purification and immobilization of recombinant proteins, (c) functionalization of biomaterials and (d) development of microarrays and probes.

Keywords
Carbohydrate-binding modules; Heterologous expression systems; Recombinant CBM-fusions; Carbohydrate-binding activity; Cellulose; CBM applications
First Page Preview
Recombinant CBM-fusion technology — Applications overview
Publisher
Database: Elsevier - ScienceDirect
Journal: Biotechnology Advances - Volume 33, Issues 3–4, May–August 2015, Pages 358–369
Authors
, , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering