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Imaging cells and sub-cellular structures with ultrahigh resolution full-field X-ray microscopy

Paper ID Volume ID Publish Year Pages File Format Full-Text
14259 1198 2013 12 PDF Available
Title
Imaging cells and sub-cellular structures with ultrahigh resolution full-field X-ray microscopy
Abstract

Our experimental results demonstrate that full-field hard-X-ray microscopy is finally able to investigate the internal structure of cells in tissues. This result was made possible by three main factors: the use of a coherent (synchrotron) source of X-rays, the exploitation of contrast mechanisms based on the real part of the refractive index and the magnification provided by high-resolution Fresnel zone-plate objectives. We specifically obtained high-quality microradiographs of human and mouse cells with 29 nm Rayleigh spatial resolution and verified that tomographic reconstruction could be implemented with a final resolution level suitable for subcellular features. We also demonstrated that a phase retrieval method based on a wave propagation algorithm could yield good subcellular images starting from a series of defocused microradiographs. The concluding discussion compares cellular and subcellular hard-X-ray microradiology with other techniques and evaluates its potential impact on biomedical research.

Keywords
X-ray microscopy; Phase contrast radiology; Fresnel phase zone plate; Subcellular organelle
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Imaging cells and sub-cellular structures with ultrahigh resolution full-field X-ray microscopy
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biotechnology Advances - Volume 31, Issue 3, May–June 2013, Pages 375–386
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us