fulltext.study @t Gmail

Experimental ATR device for real-time FTIR imaging of living cells using brilliant synchrotron radiation sources

Paper ID Volume ID Publish Year Pages File Format Full-Text
14263 1198 2013 6 PDF Available
Title
Experimental ATR device for real-time FTIR imaging of living cells using brilliant synchrotron radiation sources
Abstract

In this contribution we present the design of an original Attenuated Total Reflection (ATR)-based device designed for an IR microscope coupled to a FPA detector and optimized for in-vivo cell imaging. The optical element has been designed to perform real time experiments of cell biochemical processes. The device includes a manually removable Ge-crystal that guarantees an ease manipulation during the cell culture and a large flat surface to support the cell growth and the required change of the culture wells. This layout will allow performing sequential ATR IR imaging with the crystal immersed in the culture wells, minimizing contributions due to water vapors in the optical system. Using existing brilliant synchrotron radiation sources this ATR device may collect images at the surface of the Ge crystal at a sub-cellular spatial resolution with a penetration depth of the evanescent wave inside the sample of ~ 500 nm within few seconds. A brief summary of the cellular components that should be detected with such optical device is also presented.

Keywords
ATR, attenuated total reflectance; FPA, focal plane array; FTIR, Fourier-transform InfraRed; IRE, internal reflection element; NA, numerical aperture; SNR, signal-to-noise ratio; SR, synchrotron radiation; TIR, total internal reflectionFTIR imaging; Live
First Page Preview
Experimental ATR device for real-time FTIR imaging of living cells using brilliant synchrotron radiation sources
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Biotechnology Advances - Volume 31, Issue 3, May–June 2013, Pages 402–407
Authors
, , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us